High-throughput sequencing, data analysis, and validation Samples with RIN > 7.0 were selected for library construction. RNA extract integrity was determined using 2100 Bioanalyzer (Agilent Technologies, Inc., United States of America) via RNA Pico chip. A modified protocol was employed to remove genomic DNA contamination by incorporating Epicentre’s DNase I solution. Total RNA extraction was carried out with RNeasy Mini Kit (Qiagen). Harvested kidney and liver tissues were stored in RNAlater RNA Stabilization Agent (Qiagen). In brief, the macaques were sedated intramuscularly using general anaesthesia (combination of ketamine, 5–10 mg/kg and xylazine, 0.2–0.4 mg/kg) before a lethal dosage of Dolethal ® were given intravenously. Euthanisation and harvesting of organs were also performed by authorised and qualified veterinarians of DWNP.
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Visual examination showed that the macaques were free of disease. Two male conflict macaques that appeared to belong in the same family group were captured by the Department of Wildlife and National Parks (DWNP) from the state of Selangor. Furthermore, additional Malaysian cynomolgus macaque RNA-seq datasets will further furnish the cynomolgus macaque genome and transcriptome annotations and also provide a valuable asset for biomedical studies involving cynomolgus macaques.
fascicularis dataset for downstream applications. An additional 75,350,240 sequence reads were obtained from this study, supplementing the previous wild Peninsular Malaysian M. fascicularis were also sequenced with RNA-seq technology. This study describes the RNA sequencing (RNA-seq) of two tissues-kidney and liver-harvested from wild Peninsular Malaysian cynomolgus macaques, and is an extension of a previous study whereby the lymph node, spleen, and thymus transcriptomes of wild Peninsular Malaysian M. Therefore, it is vital that the genomes and transcriptomes of cynomolgus macaque NHP models originating from different geographical locations are sequenced as a reference for future biomedical research design and implementation. Cynomolgus macaque individuals from different geographical locations have been shown to exhibit genetic characteristics that varies between geographical origins. The cynomolgus macaque’s recapitulation of human physiology, genetics, and behaviour is advantageous as translational models for various studies in the biomedical field, including drug development and safety testing. fascicularis transcriptome assemblies, and provide a dataset for further downstream studies.Ĭynomolgus macaques ( Macaca fascicularis) are nonhuman primate (NHP) models significant to biomedicine due to their close evolutionary relationship with humans. The additional sequence dataset will further enrich existing M. Gene ontology functional categorisation showed that cellular process, catalytic activity, and cell part categories had the highest number of expressed genes, while the metabolic pathways category possessed the highest number of expressed genes in the KEGG pathway analysis. A total of 5473 significant differentially expressed genes were called. ResultsĪ total of 75,350,240 sequence reads were obtained via Hi-seq 2500 sequencing technology. fascicularis transcriptomes obtained in a past endeavour. The additional transcriptomic dataset will supplement the previously described Peninsular Malaysia M. fascicularis are significant nonhuman primate models in the biomedical field, owing to the macaque’s biological similarities with humans. Using high-throughput RNA sequencing technology, this study aimed to sequence the transcriptome of kidney and liver tissues harvested from Peninsular Malaysia cynomolgus macaque ( Macaca fascicularis).
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